This mouse coagulation Factor XII antigen assay is intended for the quantitative determination of total Factor XII antigen in mouse plasma. For research use only. Strip well format. Reagents for up to 96 tests.
Mouse Factor XII will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XII and XIIa will react with the antibody on the plate. After appropriate washing steps, biotin labeled anti mouse Factor XII primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of mouse Factor XII. Color development is proportional to the concentration of Factor XII in the samples.
More Details:
- Detection Target: Factor XII
- Reactivity: Mouse
- Range: 0.01-10 ng/ml
- Sensitivity: 0.005 ng/ml
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Please note – this product replaces our old product number IMFXIIKT-TOT (same product, new number).
