Mouse Receptor I For The Fc Region Of Immunoglobulin E ELISA Kit from Innovative Research is intended for the quantitative determination of Mouse Receptor I For The Fc Region Of Immunoglobulin E in biofluid samples, such as tissue homogenates, cell lysates and other biological fluids. This is a sandwich ELISA in strip well format with reagents for up to 96 tests . FceRI present in samples reacts with an anti-Mouse FceRI capture antibody coated and dried on a microtiter plate. After appropriate washing steps, an anti-FceRI detection antibody conjugated to biotin binds to the captured analyte. Excess antibody is washed away and bound antibody is then reacted with avidin conjugated to horseradish peroxidase. TMB substrate is used for color development at 450nm. Color development is directly proportional to the concentration of active FceRI in the samples. A standard calibration curve is prepared along with the samples to be measured using dilutions of Mouse FceRI. Detection Target: Mouse Receptor I For The Fc Region Of Immunoglobulin E Reactivity: Mouse Range: 0.156-10ng/mL Sensitivity: 0.069ng/mL Storage Conditions: Store all components at 4┬░C This Mouse FceRI activity assay is for the quantitative determination of Receptor I For The Fc Region Of Immunoglobulin E in samples including tissue homogenates, cell lysates and other biological fluids. For research use only. Strip well format. Reagents for up to 96 tests. For research use only. At Innovative Research, we provide reliable, consistent products that deliver reliable, consistent results.